Mouse Anti-SNCA Recombinant Antibody (TAB-0748CLV)

Customer Review

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Laura Brown
03.Dec,23

Ideal for SNCA Research

The Anti-SNCA Recombinant Antibody has been excellent for our SNCA research. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.

Emily Lewis
09.Aug,22

Reliable in Immunoassays

We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for SNCA is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.

John Carter
26.Sep,21

Consistent and Effective

This antibody has proven to be consistent and effective in our various assays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for SNCA have been particularly beneficial.

Q&As

1. Is the anti-SNCA recombinant antibody suitable for use in Western blotting?

   A: Yes, the anti-SNCA recombinant antibody (TAB-0748CLV) is suitable for use in Western blotting. It provides specific binding to SNCA, allowing for reliable detection in Western blot assays.

2. What are the storage recommendations for the anti-SNCA recombinant antibody?

   A: The recommended storage condition for the anti-SNCA recombinant antibody (TAB-0748CLV) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

3. Can the anti-SNCA recombinant antibody be used in immunoprecipitation assays?

   A: Yes, the anti-SNCA recombinant antibody (TAB-0748CLV) can be used in immunoprecipitation assays. It provides specific binding to SNCA, enabling the successful precipitation of the target protein from complex mixtures.

4. Is the anti-SNCA recombinant antibody effective in ELISA applications?

   A: Yes, the anti-SNCA recombinant antibody (TAB-0748CLV) is effective in ELISA applications. It has been validated for use in such assays and provides reliable detection of SNCA.

5. What is the optimal dilution for using the anti-SNCA recombinant antibody in immunofluorescence?

   A: The optimal dilution for using the anti-SNCA recombinant antibody (TAB-0748CLV) in immunofluorescence is typically 1:100 to 1:500. It is advisable to perform a dilution series to determine the best working concentration for your specific experimental conditions.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

1. Helwig, Michael, et al. "Neuronal hyperactivity-induced oxidant stress promotes in vivo α-synuclein brain spreading." Science advances 8.35 (2022): eabn0356. https://doi.org/10.1126/sciadv.abn0356
   This study investigated the effects of neuronal hyperactivity on the spreading of the Parkinson's disease-associated protein α-synuclein (αS) in the brain. Using a mouse model, researchers found that increased neuronal activity exacerbated oxidative and nitrative stress, leading to enhanced accumulation and aggregation of nitrated αS. The experiments demonstrated that hyperactivity significantly promoted the interneuronal transfer and brain-wide distribution of αS, suggesting a mechanistic role of oxidant stress in facilitating the protein's spread. The study employed advanced genetic and viral techniques to modulate neuronal activity and track the movement of αS across different brain regions, providing valuable insights into the pathophysiological processes underlying neurodegenerative diseases.
   Creative Biolabs played a crucial role in this research by supplying key reagents. They provided the Syn-O2 antibody (Cat#: TAB-0748CLV) used for double fluorescence labeling. This involved incubating sections with anti-RFP or mouse anti-Syn-O2, followed by anti-h-αS, and labeling with secondary antibodies conjugated with DyLight 488 or DyLight 594. These reagents were essential for the immunofluorescence analysis, enabling precise detection and visualization of protein interactions and modifications within the study.
2. Simon, Isak. "Expression & affinity analysis of recombinant RX against pathogenic α-synuclein." (2021).
   This study investigated the potential of recombinant RX protein constructs as a therapeutic approach to counteract the pathogenesis of Parkinson's disease by targeting the neurotoxic oligomers of α-synuclein. The research aimed to express, purify, and trimerize two RX protein constructs (D1 and D123mut) to study their binding affinity to α-synuclein monomers and oligomers using various methods, including ELISAs. The findings indicated that while D123 showed some binding affinity to α-synuclein oligomers in indirect ELISA, D1 and D123mut did not exhibit sufficient purity or binding capability. The study concluded that optimization of protein constructs and methods is crucial for further exploring RX as a therapeutic protein against Parkinson's disease.
   Creative Biolabs provided key reagents and products crucial for this study. Specifically, the Anti-Human SNCA Therapeutic Antibody (Syn-O2, CAT#: TAB-0748CLV) was used as a positive control in the ELISA assays to validate the binding affinity of RX constructs to α-synuclein. Additionally, Creative Biolabs' product ensured reliable comparisons and facilitated the validation process, thereby playing a significant role in the experimental procedures and overall findings of the study.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-0748CLV, RRID: AB_3111791)

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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